Structure had been given twenty five ?mol/L H

Cells were seeded at a density of 7,000 cells per well in a 96-well glass bottom black plates (In Vitro Scientific) to reach 80% confluence. 2O2 or with H2O2 and 4 mmol/L N-acetylcysteine (NAC) for 3 hours and stained with 5 ?mol/L of CellROX Orange reagent (Molecular Probes) and Hoechst (Thermo Fisher Scientific) at 37°C for 30 minutes. Continue reading